study of effective methods to break seed dormancy of henbane )hyoscyamus reticulatus l.(
نویسندگان
چکیده
henbane hyoscyamus reticulatus l. is a plant species in solanaceae family that seeds hardly germinate because of seed dormancy. in order to determine the best methods for seed dormancy breaking in henbane, a experiment was conducted as a completely randomized design with three replications. the treatments included moist pre-chilling (at 4 °c) for 7, 14, 21 and 28 days, combined treatment of gibberellic acid (500 ppm) and moist chilling at 4 °c for 7, 14, 21 and 28 days, soaking the seeds in hormones gibberellic acid, kinetin, auxin (250, 500, 750 and 1000 ppm) for 24 h, kno3 (0.1, 0.2 and 0.4%), hot water (70 and 90 °c) for 5, 10 and 15 minutes, soaking seeds under running water for 24 and 48h, soaking the seeds in sulfuric acid 70% and 90% (5, 10 and 15 minutes) and mechanical scarification with sandpaper. the results showed that seed dormancy in henbane is physiological since the highest germination percentage (96%) was obtained by using the combined treatment of gibberellic acid (500 ppm) and pre-chilling (for 25 days). in addition, moist chilling and gibberellic acid treatments -by itself- on breaking seed dormancy were remarkable in this plant as germination increased up to 40 and 81%, respectively. effects of hormones were also statistically significant on increasing seed germination but compared with the salient effect of combined chilling with gibberellic acid treatment and gibberellic acid treatment was not so remarkable. on the other hand, other applied treatments (hot water, sulfuric acid and mechanical scarification with sandpaper) had no effects on breaking seed dormancy in henbane which indicates that the type of seed dormancy in henbane is not physical or it is due to the accumulation of inhibitory substance in seed coat.
منابع مشابه
Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots
Lattice henbane (Hyoscyamus reticulatus L.) is an herbaceous, biennial plant belonging to Solanaceae family. H. reticulatus hairy roots were established from two-week-old leaves infected by A7 strain of Agrobacterium rhizogenes on solid Murashige and Skoog (MS) medium. In this study, abiotic elicitors including; Sodium silicate (Na2SiO3) with different concentrations (0, 1, 5 and 7 mM) and silv...
متن کاملQuantitative Analysis of l-Hyoscyamine in Hyoscyamus reticulatus L. by GC-MS
The routine quality control of a herbal drug preparation requires the identification and determination of the contents of the active ingredients in the product. Measurement alone is not sufficient; it is also necessary to know how objectively to evaluate the experimental results . There are 6 Hyoscyamus (Solanaceae) species growing in Turkey, none of which are endemic. Hyoscyamus (Solanaceae) s...
متن کاملA STUDY ON SEED GERMINATION OF AVENA LUDOVICIANA AND THE EFFECTIVE FACTORS IN SEED DORMANCY BREAKING
Wild oat has two seeds in a spikelet that they have different physiological behavior in regard of seed germiantion. The upper seed of spikelet is smaller and has deeper dormancy than the lower seed. The range of temperature for germination is 5˚C to 25 ˚C and optimal temperature is 15˚C. Both kinds of seeds showed the more germination rate in low temperatures so that 5 and 10˚C are better...
متن کاملGermination pretreatments to break hard-seed dormancy in Astragalus cicer L. (Fabaceae)
Conservationists often propagate rare species to improve their long-term population viability. However, seed dormancy can make propagation efforts challenging by substantially lowering seed germination. Here I statistically compare several pretreatment options for seeds of Astragalus cicer L.: unscarified controls and scarification via physical damage, hot water, acid, and hydrogen peroxide. Al...
متن کاملMethods for overcoming seed dormancy in jimsonweed (Datura stramonium L.)
This study aimed to examine dormancy-breaking of jimsonweed seeds. Seeds were collected from Hamedan. They were subjected to different treatments: (a) concentrated sulfuric acid for 1, 1.5, and 2 min; (b) hot water at 80°C and 90°C for 5 and 10 min; (c) mechanical scarification with sandpaper; (d) light exposure for 10, 20, and 30 days; and (e) fluctuating temperature (5–15°C). The highest germ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
پژوهش های زراعی ایرانجلد ۱۲، شماره ۲، صفحات ۲۴۶-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023